Rat HMGB-1(High Mobility Group Protein B1) ELISA Kit
中文名称：大鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠HMGB-1会与包被抗体结合。后依次加入生物素化的抗大鼠HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗大鼠HMGB-1抗体与结合在包被抗体上的大鼠HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠HMGB-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Iodine-131 radioembolization boosts the immune activation enhanced by icaritin/resiquimod in hepatocellular carcinoma
IF: 10.500 -
Free total rhubarb anthraquinones protect intestinal mucosal barrier of SAP rats via inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathway
IF: 5.400 -
Carbon dots in fried breadcrumbs: in vitro toxicity to normal rat kidney cells and its control method
IF: 4.400 -
The role of HMGB1 on SiC NPs-induced inflammation response in lung epithelial-macrophage co-culture system
IF: 3.900 -
Relationship between high-mobility group box-l and cognitive impairments induced by myocardial ischemia-reperfusion in elderly rats
IF: 3.300Journal： EXPERIMENTAL GERONTOLOGY (2024)Reactivity：RatSample Type：hippocampus tissue,Serum -
Acute hyperglycemia exacerbates neuroinflammation and cognitive impairment in sepsis-associated encephalopathy by mediating the ChREBP/HIF-1α pathway
IF: 2.800Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2024)Reactivity：RatSample Type：Serum -
Polygala tenuifolia root extract attenuates ischemic stroke by inhibiting HMGB1 trigger neuroinflammation
IF: 2.500 -
A Novel Machine Perfusion System for Enhancing Hepatic Microcirculation Perfusion
IF: 2.200 -
Activating α7nAChR suppresses systemic inflammation by mitigating neuroinflammation of the medullary visceral zone in sepsis in a rat model
IF: 1.800Journal： Translational Neuroscience (2024)Reactivity：RatSample Type：serum -
Medication overuse headache is associated with elevated lipopolysaccharide binding protein and pro-inflammatory molecules in the bloodstream
IF: 7.400
实验操作视频
