Rat HO1(Heme Oxygenase 1) ELISA Kit
中文名称：大鼠血红素氧合酶1(HO1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HO1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠HO1会与包被抗体结合。后依次加入生物素化的抗大鼠HO1抗体和辣根过氧化物酶标记的亲和素，抗大鼠HO1抗体与结合在包被抗体上的大鼠HO1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HO1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HO1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠HO1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The anti-inflammatory properties of green tea extract protect against gentamicin-induced kidney injury
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：plasma -
Role of Anonychium africanum (Plantae, Fabaceae) in Metal Oxido-Inflammatory Response: Protection Evidence in Gonad of Male Albino Rat
IF: 6.000 -
Potential neuroprotective and autophagy-enhancing effects of alogliptin on lithium/pilocarpine-induced seizures in rats: Targeting the AMPK/SIRT1/Nrf2 axis
IF: 5.200Journal： LIFE SCIENCES (2024)Reactivity：RatSample Type：hippocampal tissue -
Exosomal miR-122, miR-128, miR-200, miR-298, and miR-342 as novel diagnostic biomarkers in NAFL/NASH: Impact of LPS/TLR-4/FoxO3 pathway
IF: 5.100 -
Piracetam mitigates nephrotoxicity induced by cisplatin via the AMPK-mediated PI3K/Akt and MAPK/JNK/ERK signaling pathways
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：RatSample Type：renal tissue -
Ipomoea carnea mitigates ethanol-induced ulcers in irradiated rats via Nrf2/HO?1 pathway: an in vivo and in silico study
IF: 4.600 -
Repurposing of erythropoietin as a neuroprotective agent against methotrexate-induced neurotoxicity in rats
IF: 4.500Journal： JOURNAL OF PSYCHOPHARMACOLOGY (2024)Reactivity：RatSample Type：hippocampal tissue -
Repurposing Dapagliflozin for Mitigation of the Kidney Injury Triggered by Cadmium in Rats: Role of Autophagy, Apoptosis, and the SIRT1/Nrf2/HO-1 Pathway
IF: 4.300 -
Silymarin nanoparticles counteract cognitive impairment induced by doxorubicin and cyclophosphamide in rats; Insights into mitochondrial dysfunction and Nrf-2/HO-1 axis
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：hippocampi tissue,prefrontal cortices tissue -
Testicular toxicity in cisplatin-treated Wistar rats is mitigated by Daflon and associated with modulation of Nrf2/HO-1 and TLR4/NF-kB signaling
IF: 3.600Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)Reactivity：RatSample Type：Testicular tissue
实验操作视频
