Rat HO1(Heme Oxygenase 1) ELISA Kit
中文名称：大鼠血红素氧合酶1(HO1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HO1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠HO1会与包被抗体结合。后依次加入生物素化的抗大鼠HO1抗体和辣根过氧化物酶标记的亲和素，抗大鼠HO1抗体与结合在包被抗体上的大鼠HO1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HO1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HO1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠HO1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Carveol a Naturally-Derived Potent and Emerging Nrf2 Activator Protects Against Acetaminophen-Induced Hepatotoxicity
IF: 5.811Journal： Frontiers in Pharmacology (2021)PMID：33597885Reactivity：MouseSample Type：Tissue homogenate -
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats
IF: 4.473Journal： INFLAMMOPHARMACOLOGY (2021)PMID：33459879Reactivity：RatSample Type：Tissue homogenate(hippocampus) -
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats
IF: 4.432Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2021)PMID：33745959Reactivity：RatSample Type：Tissue homogenate(Joint Tissue) -
The potential relationship between gasotransmitters and oxidative stress, inflammation and apoptosis in lead-induced hepatotoxicity in rats
IF: 2.466 -
Potent Natural Antioxidant Carveol Attenuates MCAO-Stress Induced Oxidative, Neurodegeneration by Regulating the Nrf-2 Pathway
IF: 3.707Journal： Frontiers in Neuroscience (2020)PMID：32714135Reactivity：RatSample Type：Tissue homogenate -
New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling
IF: 3.647 -
Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：32673753Reactivity：RatSample Type：Serum,Tissue homogenate -
Activation of pCREB/Nrf-2 signaling mediates re-positioning of liraglutide as hepato-protective for methotrexate -induced liver injury (MILI)
IF: 3.775 -
The interrelationship between gasotransmitters and lead-induced renal toxicity in rats
IF: 3.499 -
Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of keap-1/nrf-2 pathway.
IF: 3.170Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2019)PMID：31039346Reactivity：RatSample Type：liver tissue
实验操作视频
