Rat IFN-γ(Interferon Gamma) ELISA Kit
中文名称：大鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗大鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗大鼠IFN-γ抗体与结合在包被抗体上的大鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway
IF: 2.809Journal： Computational and Mathematical Methods in Medicine (2022)PMID：35082916Reactivity：RatSample Type：hippocampal tissue -
Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response
IF: 2.623 -
Orally fed EGCG coronate food released TiO2 and enhanced penetrability into body organs via gut
Journal： Biomaterials Advances (2022)Reactivity：RatSample Type：Sera -
Reducing PD-L1 expression with a self-assembled nanodrug: an alternative to PD-L1 antibody for enhanced chemo-immunotherapy
IF: 11.556 -
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa
IF: 5.340 -
Protective effects of hesperidin through attenuation of Ki67 expression against DMBA-induced breast cancer in female rats
IF: 5.037 -
Effects of catechin on a rodent model of autism spectrum disorder: implications for the role of nitric oxide in neuroinflammatory pathway
IF: 4.530 -
Metformin attenuates sepsis-induced neuronal injury and cognitive impairment
IF: 3.288 -
Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34584563Reactivity：RatSample Type：serum -
Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo
IF: 5.085
实验操作视频
