Rat IFN-γ(Interferon Gamma) ELISA Kit
中文名称：大鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗大鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗大鼠IFN-γ抗体与结合在包被抗体上的大鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Curcumin combined with Baicalin attenuated ethanol-induced hepatitis by suppressing p38 MAPK and TSC1/ eIF-2α/ATF4 pathways in rats
IF: 3.067Journal： Food Bioscience (2020)Reactivity：RatSample Type：liver tissue lysate -
The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats
IF: 2.283 -
The dynamic expression of canonical Wnt/β-catenin signalling pathway in the pathologic process of experimental autoimmune neuritis
IF: 2.107Journal： INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020)PMID：32009498Reactivity：RatSample Type：sciatic nerves -
Macleaya cordata extracts exert antiviral effects in newborn mice with rotavirus?induced diarrhea via inhibiting the JAK2/STAT3 signaling pathway
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32742353Reactivity：MouseSample Type：Feces -
Nisin reduces uterine inflammation in rats by modulating concentrations of pro- and anti-inflammatory cytokines
IF: 3.091Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019)PMID：30681748Reactivity：RatSample Type：Serum -
Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats
IF: 2.276Journal： TOXICOLOGY MECHANISMS AND METHODS (2019)PMID：30426860Reactivity：RatSample Type：cardiac tissues,renal tissues -
MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis
IF: 3.967 -
Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome
IF: 3.923 -
Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity
IF: 3.457 -
Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest
IF: 3.382
实验操作视频
