Rat IgE(Immunoglobulin E) ELISA Kit
中文名称：大鼠免疫球蛋白E(IgE)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Rat
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IgE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IgE会与包被抗体结合。后依次加入生物素化的抗大鼠IgE抗体和辣根过氧化物酶标记的亲和素，抗大鼠IgE抗体与结合在包被抗体上的大鼠IgE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IgE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IgE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IgE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Long-acting anti-inflammatory injectable DEX-Gel with sustained release and self-healing properties regulates TH1/TH2 immune balance for minimally invasive treatment of allergic rhinitis
IF: 10.200Journal： JOURNAL OF NANOBIOTECHNOLOGY (2024)PMID：38321547Reactivity：RatSample Type：nasal lavage fluid (NALF)Assessing the impact of insect protein sources on intestinal health and disease: insights from human ex vivo and rat in vivo models
IF: 5.100Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：SerumMetabolomics combined with network pharmacology reveals anti-asthmatic effects of Nepeta bracteata on allergic asthma rats
IF: 3.800Journal： Chinese Herbal Medicines (2024)Reactivity：RatSample Type：serumLipidomic profiling of amniotic fluid reveals aberrant fetal lung development and fetal growth disrupted by lipid disorders during gestational asthma
IF: 3.100Journal： JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2024)Reactivity：RatSample Type：SerumGreen tea polyphenols alleviate β-conglycinin-induced anaphylaxis by modulating gut microbiota in rats
IF: 5.200Ephedra Herb extract ameliorates adriamycin-induced nephrotic syndrome in rats via the CAMKK2/AMPK/mTOR signaling pathway
IF: 4.600Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat
IF: 3.759Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway inflammation in rats
IF: 3.838Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma
IF: 3.743Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)PMID：31315072Reactivity：RatSample Type：Serum,Tissue homogenate
