Rat IL-10(Interleukin 10) ELISA Kit
中文名称：大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Amphiphilic Gelator-Based Shear-Thinning Hydrogel for Minimally Invasive Delivery via Endoscopy Catheter to Remove Gastrointestinal Polyps
IF: 13.000 -
A novel multifunctional nanocomposite hydrogel orchestrates the macrophage reprogramming-osteogenesis crosstalk to boost bone defect repair
IF: 10.600 -
Oxygen-generating hydrogels combined with electrical stimulation: A dual approach for promoting diabetic wound healing
IF: 9.400Journal： Acta Biomaterialia (2024)Reactivity：RatSample Type：wound tissue -
Chitosan-based self-healing thermosensitive hydrogel loaded with siHMGB1 for treatment of rheumatoid arthritis via macrophage repolarization
IF: 7.700Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：RatSample Type：serum -
Exploring the mechanism of Paotianxiong polysaccharide in the treatment of chronic kidney disease combining metabolomics and microbiomics technologies
IF: 7.700Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：RatSample Type：serum -
BMP2 enhance the osteogenic effect of BMSCs-derived exosomes in skull defect of diabetic rats
IF: 7.600 -
Dihydromyricetin treats pulmonary hypertension by modulating CKLF1/CCR5 axis-induced pulmonary vascular cell pyroptosis
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：serum -
IRF1 governs the expression of SMARCC1 via the GCN5-SETD2 axis and actively engages in the advancement of osteoarthritis
IF: 6.600Journal： Journal of Orthopaedic Translation (2024)Sample Type：chondrocytes -
High-intensity interval training mitigates the progression of periodontitis and improves behavioural aspects in rats
IF: 5.800Journal： JOURNAL OF CLINICAL PERIODONTOLOGY (2024)Reactivity：RatSample Type：blood -
Placental mesenchymal stem cells suppress inflammation and promote M2-like macrophage polarization through the IL-10/STAT3/NLRP3 axis in acute lung injury
IF: 5.700Journal： Frontiers in Immunology (2024)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF)
实验操作视频
