Rat IL-10(Interleukin 10) ELISA Kit
中文名称：大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Biochemical and histological alterations of doxorubicin-induced neurotoxicity in rats: Protective role of luteolin
IF: 3.652Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021)PMID：34766659Reactivity：RatSample Type：brain -
Germinated millet flour (Pennisetum glaucum (L.) R. Br.) reduces inflammation, oxidative stress, and liver steatosis in rats fed with high-fat high-fructose diet
IF: 3.616Journal： JOURNAL OF CEREAL SCIENCE (2021)Reactivity：ratSample Type：Blood -
Effects of hydroxysafflor yellow A on rats with collagen-induced arthritis
IF: 3.575Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021)PMID：34271433Reactivity：RatSample Type：serum -
Chlorogenic acid abates oxido-inflammatory and apoptotic responses in the liver and kidney of Tamoxifen-treated rats
IF: 3.524 -
The modulatory effect of taurine on benzo (a) pyrene-induced hepatorenal toxicity
IF: 3.524 -
Celastrol Attenuates Learning and Memory Deficits in an Alzheimer’s Disease Rat Model
IF: 3.411 -
Rupatadine protects the intestinal mucosa from injury by 5-flurouracil via modulation of inflammation, apoptosis and intestinal permeability
IF: 3.356 -
Bromelain protects against cisplatin-induced ocular toxicity through mitigating oxidative stress and inflammation
IF: 3.356Journal： DRUG AND CHEMICAL TOXICOLOGY (2021)Reactivity：RatSample Type：ocular tissue -
Luteolin attenuates doxorubicin-induced derangements of liver and kidney by reducing oxidative and inflammatory stress to suppress apoptosis
IF: 2.903Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2021)PMID：33827303Reactivity：RatSample Type：Tissue homogenate -
Chlorogenic acid co-administration abates tamoxifen-mediated reproductive toxicities in male rats: An experimental approach
IF: 2.720Journal： JOURNAL OF FOOD BIOCHEMISTRY (2021)PMID：33491243Reactivity：RatSample Type：Tissue homogenate
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