Rat IL-10(Interleukin 10) ELISA Kit
中文名称：大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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C1q/TNF-related protein-9 promotes macrophage polarization and improves cardiac dysfunction after myocardial infarction
IF: 4.522Journal： JOURNAL OF CELLULAR PHYSIOLOGY (2019)PMID：30953351Reactivity：RatSample Type：Tissue homogenate -
Accelerated Blood Clearance of Nanoemulsions Modified with PEG-Cholesterol and PEG-Phospholipid Derivatives in Rats: The Effect of PEG-Lipid Linkages and PEG Molecular Weights
IF: 4.396 -
Deep-Fried Atractylodis Rhizoma Protects against Spleen Deficiency-Induced Diarrhea through Regulating Intestinal Inflammatory Response and Gut Microbiota
IF: 4.183Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)PMID：31878055Reactivity：RatSample Type：serum -
Effects of IL-10- and FasL-overexpressing dendritic cells on liver transplantation tolerance in a heterotopic liver transplantation rat model
IF: 3.947Journal： IMMUNOLOGY AND CELL BIOLOGY (2019)PMID：30977930Reactivity：RatSample Type：Cell culture supernatant -
Docosahexaenoic Acid Increases the Potency of Soluble Epoxide Hydrolase Inhibitor in Alleviating Streptozotocin-Induced Alzheimer’s Disease-Like Complications of Diabetes
IF: 3.845Journal： Frontiers in Pharmacology (2019)PMID：31068802Reactivity：RatSample Type：hippocampus tissues -
Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study
IF: 3.448Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2019)PMID：31099131Reactivity：RatSample Type：Tissue homogenate -
Therapeutic effect of treatment with metformin and/or 4-hydroxychalcone in male Wistar rats with nonalcoholic fatty liver disease
IF: 3.170 -
Nisin reduces uterine inflammation in rats by modulating concentrations of pro- and anti-inflammatory cytokines
IF: 3.091Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019)PMID：30681748Reactivity：RatSample Type：Serum -
Changes in alpha-7 nicotinic acetylcholine receptor and macrophage polarization state participate in the regulation of cervical remodeling in pregnant rats
IF: 2.960 -
Effects of depression on healing and inflammatory responses of acute wounds in rats
IF: 2.850
实验操作视频
