Rat IL-10(Interleukin 10) ELISA Kit
中文名称：大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Gastrodin alleviates perioperative neurocognitive dysfunction of aged mice by suppressing neuroinflammation
IF: 3.263Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2020)PMID：33220272Reactivity：RatSample Type：Tissue homogenate(Hippocampal tissues) -
KGF Is Delivered to Inflammatory and Induces the Epithelial Hyperplasia in Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis Rats
IF: 3.216Journal： Drug Design Development and Therapy (2020)PMID：32021106Reactivity：RatSample Type：intestinal tissue -
The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572927Reactivity：RatSample Type：Serum -
The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model
IF: 2.907 -
Hydrogen sulfide renal protective effects: possible link between hydrogen sulfide and endogenous carbon monoxide in a rat model of renal injury
IF: 2.892 -
Inhibition of the fatty acid amide hydrolase changes behaviors and brain catecholamines in a sex-specific manner in rats exposed to chronic unpredictable stress
IF: 2.826 -
Agomelatine prevents indomethacin-induced gastric ulcer in rats
IF: 2.754 -
Effects of co-administration of Ganoderma terpenoid extract with chloroquine on inflammatory markers and antioxidant status in Plasmodium berghei-infected mice
IF: 2.446 -
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
IF: 2.146Journal： Iranian Journal of Basic Medical Sciences (2020)PMID：33149866Reactivity：RatSample Type：Tissue homogenate -
Protective effect of Alpinetin on rats with chronic obstructive pulmonary disease
IF: 1.797Journal： Food Science & Nutrition (2020)PMID：33312544Reactivity：RatSample Type：Cell culture supernatant
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