Rat IL-10(Interleukin 10) ELISA Kit
中文名称：大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Combination Therapy with Vitamin D and Metformin: A Potential Approach to Mitigate Testicular Dysfunction in Type 2 Diabetes Mellitus
IF: 2.600Journal： Reproductive Sciences (2024)Reactivity：RatSample Type：Testicular tissue -
Preventive effects of melatonin and amifostine on irradiated rats with experimental periodontitis
IF: 2.600Journal： BMC Oral Health (2024)Reactivity：RatSample Type：periodontal tissue -
Effects of mid?gestational sevoflurane and magnesium sulfate on maternal oxidative stress, inflammation and fetal brain histopathology
IF: 2.400Journal： Experimental and Therapeutic Medicine (2024)Reactivity：RatSample Type：serum -
Formulated Chitosan Microspheres Remodelled the Altered Gut Microbiota and Liver miRNA in Diet-Induced Type-2 Diabetic Rats
IF: 2.400Journal： CARBOHYDRATE RESEARCH (2024)Reactivity：RatSample Type：serum -
Physical exercise improved the hematological effect of vitamin D in type 2 diabetes mellitus-induced nephrotoxicity in rats
IF: 2.300Journal： Biochemistry and Biophysics Reports (2024)Reactivity：RatSample Type：Renal tissue -
Antioxidant and inflammatory-modulating properties of ginger and bitterleaf teas
IF: 2.200Journal： INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2024)Reactivity：RatSample Type：plasma -
A Novel Machine Perfusion System for Enhancing Hepatic Microcirculation Perfusion
IF: 2.200 -
Pancreastatin inhibitor PSTi8 improves ovarian health in Letrozole-HFD induced PCOS rats by ameliorating metabolic and reproductive parameters
IF: 2.100 -
A bitter flavonoid gum from Dorema aucheri accelerate wound healing in rats: Involvement of Bax/HSP 70 and hydroxyprolin mechanisms
IF: 2.000 -
Synergistic Effects of Vitamin D and Exercise on Diabetes-induced Gonadotoxicity in Male Wistar Rats: Role of Xanthine Oxidase/Uric Acid and Nrf2/NfkB Signaling
IF: 1.800Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)Reactivity：RatSample Type：Testicular tissue
实验操作视频
