Rat IL-10(Interleukin 10) ELISA Kit
中文名称:大鼠白介素10(IL-10)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-10抗体包被于酶标板上,实验时样品(或标准品)中的大鼠IL-10会与包被抗体结合。后依次加入生物素化的抗大鼠IL-10抗体和辣根过氧化物酶标记的亲和素,抗大鼠IL-10抗体与结合在包被抗体上的大鼠IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Autophagy Improves Inflammatory Response in Sepsis Accompanied by Changes in Gut Microbiota
IF: 4.4 -
Genetically engineered electrospinning contributes to spinal cord injury repair by regulating the immune microenvironment
IF: 4.3Journal: Frontiers in Bioengineering and Biotechnology (2024)Reactivity:Rat -
Transcriptomic Insights into Different Stimulation Intensity of Electroacupuncture in Treating COPD in Rat Models
IF: 4.2Journal: Journal of Inflammation Research (2024)Reactivity:RatSample Type:serum -
Agmatine modulation of gut-brain axis alleviates dysbiosis-induced depression-like behavior in rats
IF: 4.2Journal: EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity:RatSample Type:prefrontal cortex (PFC),hippocampus tissue -
Neuroprotective effect of diosmin against chlorpyrifos-induced brain intoxication was mediated by regulating PPAR-γ and NF-κB/AP-1 signals
IF: 3.9Journal: FOOD AND CHEMICAL TOXICOLOGY (2024)Reactivity:RatSample Type:brain tissue -
Neuroinflammation in the medullary visceral zone exert a powerful impaction on the systemic inflammation in sepsis through cholinergic anti-inflammatory pathway
IF: 3.8 -
Effects of apelin on neonatal brain neurogenesis in L-NAME-induced maternal preeclampsia
IF: 3.8Journal: Scientific Reports (2024)Reactivity:RatSample Type:brain tissue -
Brazil nut (Bertholletia excelsa) and metformin abrogate cardiac complication in fructose/STZ-induced type 2 diabetic rats by attenuating oxidative stress and modulating the MAPK-mTOR/NFkB/IL-10 signaling pathways
IF: 3.5Journal: Food & Nutrition Research (2024)Reactivity:RatSample Type:heart tissue -
Effects of Moleac 901 after severe spinal cord injury on chronic phase in Wistar rats
IF: 3.4Journal: Heliyon (2024)Reactivity:RatSample Type:spinal cord tissue -
Huang Lian Jie Du decoction attenuates inflammation in septic rats by activating autophagy and altering the intestinal microbiome
IF: 3.4

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