Rat IL-17A(Interleukin 17A) ELISA Kit
中文名称：大鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗大鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-17A抗体与结合在包被抗体上的大鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Supramolecular gel with enhanced immunomodulatory effects presents a minimally invasive treatment strategy for eosinophilic chronic rhinosinusitis
IF: 10.500Journal： JOURNAL OF CONTROLLED RELEASE (2024)Reactivity：RatSample Type：nasal lavage fluid -
Anthocyanin-rich black wheat as a functional food for managing type 2 diabetes mellitus: a study on high fat diet-streptozotocin-induced diabetic rats
IF: 5.100 -
Xuetongsu Ameliorates Synovial Inflammatory Hyperplasia in Rheumatoid Arthritis by Inhibiting JAK2/STAT3 and NF-κB Signaling Pathways
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Wenyang-Tianjing-Jieyu Decoction Improves Depression Rats of Kidney Yang Deficiency Pattern by Regulating T Cell Homeostasis and Inflammation Level
IF: 3.200Journal： Neuropsychiatric Disease and Treatment (2024)PMID：38545129Reactivity：RatSample Type：hippocampus tissue -
Mechanistic analyses reveal that Pueraria montana var. lobata (Willd.) is effective in inhibiting ovarian cancer progression
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Nanotechnology strategy for inhibition of PARP1 and IL-17A-associated with neurotoxicity in rats exposed to hospital wastewater
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：brain tissue -
Formulated Chitosan Microspheres Remodelled the Altered Gut Microbiota and Liver miRNA in Diet-Induced Type-2 Diabetic Rats
IF: 2.400Journal： CARBOHYDRATE RESEARCH (2024)Reactivity：RatSample Type：serum -
Fecal microbiota transplantation as a potential therapeutic approach to improve impaired glucose tolerance via gut microbiota modulation in rat model
IF: 1.800Journal： Journal of Diabetes and Metabolic Disorders (2024)Reactivity：RatSample Type：Serum -
Medication overuse headache is associated with elevated lipopolysaccharide binding protein and pro-inflammatory molecules in the bloodstream
IF: 7.400 -
Palmatine treats urticaria by reducing inflammation and increasing autophagy
IF: 7.300
实验操作视频
