Rat IL-17A(Interleukin 17A) ELISA Kit
中文名称：大鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗大鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-17A抗体与结合在包被抗体上的大鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Thermosensitive smart hydrogel of PEITC ameliorates the therapeutic efficacy in rheumatoid arthritis
IF: 5.112Journal： EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022)PMID：36572358Reactivity：RatSample Type：serum -
Extracellular vesicles ?produced?by?bone marrow mesenchymal stem cells overexpressing programmed death-ligand 1 ameliorate dextran sodium sulfate-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis
IF: 4.369Journal： JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022)PMID：36044618Reactivity：RatSample Type：blood, colon -
Platelet-rich plasma exhibits anti-inflammatory effect and attenuates cardiomyocyte damage by reducing NF-κB and enhancing VEGF expression in isoproterenol induced cardiotoxicity model
IF: 4.109 -
Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance
IF: 3.464 -
Effect of moxibustion on autophagy and the inflammatory response of synovial cells in rheumatoid arthritis model rat.
IF: 2.547Journal： Journal of Traditional Chinese Medicine (2022)Reactivity：RatSample Type：serum -
Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine
IF: 2.363Journal： Metabolic Syndrome and Related Disorders (2022)PMID：35796694Reactivity：RatSample Type：Liver tissue -
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa
IF: 5.340 -
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways
IF: 4.360 -
Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage
IF: 4.219 -
Imbalanced inflammatory response in subchronic arsenic-induced liver injury and the protective effects of Ginkgo biloba extract in rats: Potential role of cytokines mediated cell–cell interactions
IF: 4.119
实验操作视频
