Rat IL-18(Interleukin 18) ELISA Kit
中文名称：大鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-18会与包被抗体结合。后依次加入生物素化的抗大鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-18抗体与结合在包被抗体上的大鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Enriched Environment Attenuates Pyroptosis to Improve Functional Recovery After Cerebral Ischemia/Reperfusion Injury
IF: 5.750Journal： Frontiers in Aging Neuroscience (2021)PMID：34646128Reactivity：RatSample Type：penumbra tissue -
Folic Acid Alleviates High Glucose and Fat-Induced Pyroptosis via Inhibition of the Hippo Signal Pathway on H9C2 Cells
IF: 5.246 -
Fisetin attenuates periodontitis through FGFR1/TLR4/NLRP3 inflammasome pathway
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33725636Reactivity：RatSample Type：serum,Cell culture supernatant(THP-1 cells) -
Pinocembrin inhibited cardiomyocyte pyroptosis against doxorubicin-induced cardiac dysfunction via regulating Nrf2/Sirt3 signaling pathway
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33752080Reactivity：RatSample Type：Serum,Cell culture supernatant -
Suppression of NLRP3 inflammasome by Platycodin D via the TLR4/MyD88/NF-κB pathway contributes to attenuation of lipopolysaccharide induced acute lung injury in rats
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33872850Reactivity：RatSample Type：Bronchoalveolar Lavage Fluid -
Exploring the role of Aquaporins (AQPs) in LPS induced systemic inflammation and the ameliorative effect of Garcinia in male Wistar rat
IF: 4.473 -
Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats
IF: 4.119 -
Nicotinamide mononucleotide attenuates doxorubicin-induced cardiotoxicity by reducing oxidative stress, inflammation and apoptosis in rats
IF: 4.013Journal： ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2021)PMID：34610336Reactivity：RatSample Type：plasma, heart -
Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway
IF: 3.906 -
Moxibustion against Cyclophosphamide-Induced Premature Ovarian Failure in Rats through Inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent Pyroptosis
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：33613687Reactivity：RatSample Type：Serum
实验操作视频
