Rat IL-18(Interleukin 18) ELISA Kit
中文名称：大鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-18会与包被抗体结合。后依次加入生物素化的抗大鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-18抗体与结合在包被抗体上的大鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Proteomic Analysis and In Vivo Studies Reveal the Potential Gastroprotective Effects of CHCl3 and Aqueous Extracts of Ficus palmata
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34194521Reactivity：RatSample Type：Tissue homogenate -
Emodin ameliorates acute pancreatitis?induced?lung injury by suppressing NLRP3 inflammasome?mediated neutrophil recruitment
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34178130Reactivity：RatSample Type：serum -
Activated protein C overexpression suppresses the pyroptosis of subarachnoid hemorrhage model cells by regulating the NLRP3 inflammasome pathway
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34650639Reactivity：RatSample Type：brain tissue -
Sodium para-aminosalicylic acid inhibits manganese-induced NLRP3 inflammasome-dependent pyroptosis by inhibiting NF-κB pathway activation and oxidative stress
IF: 5.793Journal： Journal of Neuroinflammation (2020)PMID：33203418Reactivity：RatSample Type：Tissue homogenate -
Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia
IF: 5.076Journal： Oxidative Medicine and Cellular Longevity (2020)PMID：33294118Reactivity：RatSample Type：Cell culture medium -
Salidroside ameliorates Parkinson's disease by inhibiting NLRP3-dependent pyroptosis
IF: 4.831Journal： Aging-US (2020)PMID：32432571Reactivity：Mouse,RatSample Type：Cell culture supernatant,Tissue homogenate -
Palmitic acid stimulates NLRP3 inflammasome activation through TLR4-NF-κB signal pathway in hepatic stellate cells
IF: 3.297Journal： Annals of Translational Medicine (2020)PMID：32309315Reactivity：RatSample Type：Serum,Cell culture supernatant,Tissue homogenate -
Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats
IF: 3.139Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2020)PMID：32493122Reactivity：RatSample Type：Tissue homogenate -
Methoxytetrahydro-2H-pyran-2-yl)methyl benzoate inhibits spinal cord injury in the rat model via PPAR-γ/PI3K/p-Akt activation
IF: 3.118Journal： ENVIRONMENTAL TOXICOLOGY (2020)PMID：32149473Reactivity：RatSample Type：spinal cord tissue -
MiRNA-1 promotes pyroptosis of cardiomyocytes and release of inflammatory factors by downregulating the expression level of PIK3R1 through the FoxO3a pathway
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：33215443Reactivity：Rat
实验操作视频
