Rat IL-18(Interleukin 18) ELISA Kit
中文名称：大鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-18会与包被抗体结合。后依次加入生物素化的抗大鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-18抗体与结合在包被抗体上的大鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
KAT3B-mediated succinylation of DERL3 suppresses osteogenic differentiation by promoting M1/M2 macrophage polarization
IF: 5.3Journal： BIOCHEMICAL PHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Bay 11-7082 mitigates oxidative stress and mitochondrial dysfunction via NLRP3 inhibition in experimental diabetic neuropathy
IF: 5.2Journal： LIFE SCIENCES (2024)Reactivity：RatSample Type：Sciatic nerve tissue -
Pramipexole improves depression-like behavior in diabetes mellitus with depression rats by inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing impaired neuroplasticity
IF: 4.9Journal： JOURNAL OF AFFECTIVE DISORDERS (2024)Reactivity：RatSample Type：serum -
Bergapten attenuates hemorrhagic shock induced multi-organ injury by inhibiting NLRP3 inflammasome activation and pyroptosis
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：RatSample Type：renal tissue -
Tangzu granule alleviate neuroinflammation in diabetic peripheral neuropathy by suppressing pyroptosis through P2X7R /NLRP3 signaling pathway
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：RatSample Type：sciatic nerve,serum -
Butyrate attenuates intestinal inflammation in Crohn’s disease by suppressing pyroptosis of intestinal epithelial cells via the cGSA-STING-NLRP3 axis
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：Rat,HumanSample Type：colon tissue -
Endplate chondrocyte-derived exosomal miR-128-3p mitigates intervertebral disc degeneration by targeting TRAF6 via the miR-128-3p/TRAF6 axis to suppress pyroptosis
IF: 4.8 -
New idea of Fuke Qianjin capsule in treating sequelae of pelvic inflammatory disease: anti-inflammatory in the early stage and reparative in the later stage
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Integrated Network Pharmacology Analysis and Experimental Validation to Elucidate the Mechanism of Acteoside in Treating Diabetic Kidney Disease
IF: 4.7Journal： Drug Design Development and Therapy (2024)Reactivity：RatSample Type：kidney tissues -
Autophagy alleviates hippocampal neuroinflammation by inhibiting the NLRP3 inflammasome in a juvenile rat model exposed particulate matter
IF: 4.5
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