Rat IL-18(Interleukin 18) ELISA Kit
中文名称:大鼠白介素18(IL-18)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-18抗体包被于酶标板上,实验时样品(或标准品)中的大鼠IL-18会与包被抗体结合。后依次加入生物素化的抗大鼠IL-18抗体和辣根过氧化物酶标记的亲和素,抗大鼠IL-18抗体与结合在包被抗体上的大鼠IL-18结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-18浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-18的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠IL-18,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
TFEB attenuates hyperglycemia-induced retinal capillary endothelial cells injury via autophagy regulation
IF: 3.900 -
Acetaldehyde dehydrogenase 2 activation attenuates sepsis-induced brain injury through NLRP3 inflammasome regulation
IF: 3.800 -
Hydrogen sulfide alleviates ischemia induced liver injury by repressing the SPHK1/S1P pathway
IF: 3.616 -
Pharmacological evaluation of carvacrol anti-migraine potential
IF: 3.600Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID:36723608Reactivity:RatSample Type:brain -
High-dose remifentanil exacerbates myocardial ischemia-reperfusion injury through activation of calcium-sensing receptor-mediated pyroptosis
IF: 3.6 -
Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats
IF: 3.508Journal: Journal of Personalized Medicine (2023)PMID:36983559Reactivity:RatSample Type:lung tissues -
Agathis dammara Extract and its Monomer Araucarone Attenuate Abdominal Aortic Aneurysm in Mice
IF: 3.4 -
The lncRNA HOTAIR attenuates pyroptosis of diabetic cardiomyocytes by recruiting FUS to regulate SIRT3 expression
IF: 3.300 -
Boric acid is associated with the suppression of apoptosis and endoplasmic reticulum stress in rat model of paracetamol -induced hepatotoxicity
IF: 3.3Journal: Journal of Taibah University for Science (2023)Reactivity:RatSample Type:liver,Serum -
Botulinum toxin type A ameliorates rat dorsal root ganglia neuron pyroptosis in postherpetic neuralgia by upregulating cathelicidin antimicrobial peptide to inhibit neutrophil elastase
IF: 3.0Journal: Chemical Biology & Drug Design (2023)PMID:38065687Reactivity:RatSample Type:dorsal root ganglia (DRG)

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