Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Estrogen hindrance escalates inflammation and neurodegeneration in the hippocampal regions of collagen-induced arthritis female Sprague–Dawley rats
IF: 2.900Journal： PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2024)Reactivity：RatSample Type：Serum -
Sodium acetate prevents testicular damage in Wistar rats subjected to testicular ischaemia/reperfusion injury
IF: 2.800Journal： EXPERIMENTAL AND MOLECULAR PATHOLOGY (2024)Reactivity：RatSample Type：Testicular tissue -
Acute hyperglycemia exacerbates neuroinflammation and cognitive impairment in sepsis-associated encephalopathy by mediating the ChREBP/HIF-1α pathway
IF: 2.800Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2024)Reactivity：RatSample Type：Serum -
Pulsed radiofrequency alleviates neuropathic pain by upregulating MG53 to inhibit microglial activation
IF: 2.800Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2024)Reactivity：RatSample Type：spinal cord tissue -
Synergistic Effect of Donepezil and Neurotropic B Vitamins on Dysregulated Antioxidant, Inflammation and Neurotransmitter Status in Aluminium Chloride-Induced Neurotoxicity in Rats
IF: 2.800Journal： CELL BIOCHEMISTRY AND FUNCTION (2024)Reactivity：RatSample Type：brain tissue -
Fluorofenidone attenuates renal fibrosis by inhibiting lysosomal cathepsin?mediated NLRP3 inflammasome activation
IF: 2.700Journal： Experimental and Therapeutic Medicine (2024) -
Farnesol attenuates cadmium-induced kidney injury by mitigating oxidative stress, inflammation and necroptosis and upregulating cytoglobin and PPARγ in rats
IF: 2.700Journal： TISSUE & CELL (2024)Reactivity：RatSample Type：kidney tissue -
Alantolactone improves cognitive impairment in rats with Porphyromonas gingivalis infection by inhibiting neuroinflammation, oxidative stress, and reducing Aβ levels
IF: 2.700Journal： BRAIN RESEARCH (2024)Reactivity：RatSample Type：hippocampal tissue,cortical tissue -
Attenuation of inflammation, oxidative stress and TGF-β1/Smad3 signaling and upregulation of Nrf2/HO-1 signaling mediate the protective effect of diallyl disulfide against cadmium nephrotoxicity
IF: 2.700Journal： TISSUE & CELL (2024)Reactivity：RatSample Type：Kidney tissue -
Electroacupuncture extends the time window of thrombolytic therapy in rats by reducing disruptions of blood–brain barrier and inhibiting GSDMD-mediated pyroptosis
IF: 2.700Journal： BRAIN RESEARCH (2024)Reactivity：RatSample Type：brain ischemic penumbra tissue
实验操作视频
