Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Muscone Abates 1,2-Dimethylhydrazine-Induced Colon Cancer in Rats and HCT-116 Cells Proliferation via Modulation of TNF-α, COX-2, NF-kB, and Phase I Xenobiotic Enzymes
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Effects of Turmeric, Ginger, Cinnamon, and Garlic Essential Oils on HSP 70, NF-κB, Oxidative DNA Damage, Inflammatory Cytokines, and Oxidative Markers in Broiler
IF: 1.300Journal： Translational Animal Science (2024)Reactivity：ChickenSample Type：serum -
Isoflurane preconditioning protects against renal ischemia/reperfusion injury in diabetes via activation of the Brg1/Nrf2/HO-1 signaling pathway
IF: 1.100Journal： Acta Cirurgica Brasileira (2024)Reactivity：RatSample Type：renal tissue -
Pinobanksin Attenuates Cerebral Ischemia–Reperfusion Injury in Rats Through Mitigating the Inflammatory Responses
IF: 0.600Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：Serum,Brain Tissue -
Monotropein Reduces Retinal Inflammation, Oxidative Stress, and Dyslipidemia in Streptozotocin-induced Diabetic Retinopathy in Rats
IF: 0.600Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：serum -
Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy
IF: 11.300 -
Inhibition of circulating exosomes release with GW4869 mitigates severe acute pancreatitis-stimulated intestinal barrier damage through suppressing NLRP3 inflammasome-mediated pyroptosis
IF: 5.600Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)Reactivity：RatSample Type：intestinal tissues -
Ameliorative effects of vitamins-loaded flavoured nanophytosomes fortified with star anise volatile oil against CsA-Induced liver and kidney injury in rats: Application in functional ice cream.
IF: 4.000 -
Melatonin attenuates inflammation and cardiac dysfunction in myocardial infarction by regulating the miRNA-200b-3p/high mobility group box chromosomal protein 1 axis
IF: 2.200Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：serum -
Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway
IF: 1.800Journal： GROWTH FACTORS (2023)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF)
实验操作视频
