Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Fisetin attenuates periodontitis through FGFR1/TLR4/NLRP3 inflammasome pathway
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33725636Reactivity：RatSample Type：serum,Cell culture supernatant(THP-1 cells) -
Pinocembrin inhibited cardiomyocyte pyroptosis against doxorubicin-induced cardiac dysfunction via regulating Nrf2/Sirt3 signaling pathway
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：33752080Reactivity：RatSample Type：Serum,Cell culture supernatant -
25-Hydroxycholesterol mitigates hepatic ischemia reperfusion injury via mediating mitophagy
IF: 4.932 -
Protective effect of mirtazapine against acetic acid-induced ulcerative colitis in rats: Role of NLRP3 inflammasome pathway
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：34601335Reactivity：RatSample Type：colonic tissue -
N-acetyl cysteine abates hepatorenal toxicities induced by perfluorooctanoic acid exposure in male rats
IF: 4.860Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021)PMID：33933708Reactivity：RatSample Type：Serum,Tissue homogenate -
Fecal microbiota transplantation ameliorates stress-induced depression-like behaviors associated with the inhibition of glial and NLRP3 inflammasome in rat brain
IF: 4.791Journal： JOURNAL OF PSYCHIATRIC RESEARCH (2021)PMID：33677218Reactivity：RatSample Type：cortex , hippocampus -
Olanzapine-Induced Activation of Hypothalamic Astrocytes and Toll-Like Receptor-4 Signaling via Endoplasmic Reticulum Stress Were Related to Olanzapine-Induced Weight Gain
IF: 4.677 -
Selenium abates manganese–induced striatal and hippocampal toxicity via abrogation of neurobehavioral deficits, biometal accumulation, oxidative stress, inflammation, and caspase-3 activation in rats
IF: 4.530 -
The impact of Sambucus nigra L. extract on inflammation, oxidative stress and tissue remodeling in a rat model of lipopolysaccharide-induced subacute rhinosinusitis
IF: 4.473 -
Exploring the role of Aquaporins (AQPs) in LPS induced systemic inflammation and the ameliorative effect of Garcinia in male Wistar rat
IF: 4.473
实验操作视频
