Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Inhibition of circulating exosomes release with GW4869 mitigates severe acute pancreatitis-stimulated intestinal barrier damage through suppressing NLRP3 inflammasome-mediated pyroptosis
IF: 5.6Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)Reactivity：RatSample Type：intestinal tissues -
Downregulation of ATF-4 Attenuates the Endoplasmic Reticulum Stress–Mediated Neuroinflammation and Cognitive Impairment in Experimentally Induced Alzheimer's Disease Model
IF: 5.1Journal： MOLECULAR NEUROBIOLOGY (2023)Reactivity：RatSample Type：hippocampus (HP) tissue,cerebral cortex tissue -
Ameliorative effects of vitamins-loaded flavoured nanophytosomes fortified with star anise volatile oil against CsA-Induced liver and kidney injury in rats: Application in functional ice cream.
IF: 4.0 -
Melatonin attenuates inflammation and cardiac dysfunction in myocardial infarction by regulating the miRNA-200b-3p/high mobility group box chromosomal protein 1 axis
IF: 2.2Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：serum -
Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway
IF: 1.8Journal： GROWTH FACTORS (2023)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
Antioxidant, anti-inflammatory, and anti-reprotoxic effects of kaempferol and vitamin E on lead acetate-induced testicular toxicity in male rats
IF: 1.2Journal： Open Veterinary Journal (2023)Reactivity：RatSample Type：Testicular -
The collagen enhancement by Spirulina extract in intrinsic and extrinsic skin aging in albino rat
Journal： Journal of Basic and Applied Zoology (2023)Reactivity：RatSample Type：skin tissue -
An all-silk-derived bilayer hydrogel for osteochondral tissue engineering
IF: 10.761 -
Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches
IF: 9.043 -
Effects of Malate Ringer's solution on myocardial injury in sepsis and enforcement effects of TPP@PAMAM-MR
IF: 8.440Journal： Journal of Translational Medicine (2022)Reactivity：RatSample Type：venous blood
实验操作视频
