Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Mechanistic insights into the anti-depressant effect of emodin: an integrated systems pharmacology study and experimental validation
IF: 5.682Journal： Aging-US (2021)Reactivity：RatSample Type：prefrontal cortex (PFC) tissue -
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism
IF: 5.555 -
Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo
IF: 5.455Journal： Chinese Medicine (2021)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
Inhibiting Brd4 alleviated PTSD-like behaviors and fear memory through regulating immediate early genes expression and neuroinflammation in rats
IF: 5.372Journal： JOURNAL OF NEUROCHEMISTRY (2021)PMID：34050937Reactivity：RatSample Type：Tissue homogenate -
Zinc induces reactive astrogliosis through ERK-dependent activation of Stat3 and promotes synaptic degeneration
IF: 5.372 -
The piperine derivative HJ105 inhibits Aβ1–42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis
IF: 5.340Journal： PHYTOMEDICINE (2021)PMID：33994056Reactivity：RatSample Type：Tissue homogenate(hippocampus) -
Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis
IF: 5.340 -
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa
IF: 5.340 -
Mesenchymal stem cells ameliorate silica-induced pulmonary fibrosis by inhibition of inflammation and epithelial-mesenchymal transition
IF: 5.310Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)PMID：34076355Reactivity：RatSample Type：lung,bronchoalveolar lavage fluid -
Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats
IF: 5.310
实验操作视频
