Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Limonin ameliorates acute pancreatitis by suppressing JAK2/STAT3 signaling pathway
IF: 4.119 -
Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats
IF: 4.119 -
MiR-708-3p Alleviates Inflammation and Myocardial Injury After Myocardial Infarction by Suppressing ADAM17 Expression
IF: 4.092Journal： INFLAMMATION (2021)PMID：33492555Reactivity：RatSample Type：Tissue homogenate,Cell culture supernatant -
Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo
IF: 4.092 -
Nicotinamide mononucleotide attenuates doxorubicin-induced cardiotoxicity by reducing oxidative stress, inflammation and apoptosis in rats
IF: 4.013Journal： ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2021)PMID：34610336Reactivity：RatSample Type：plasma, heart -
The beneficial activity of curcumin and resveratrol loaded in nanoemulgel for healing of burn-induced wounds
IF: 3.981Journal： JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2021)Reactivity：RatSample Type：Tissue homogenate -
Brain-derived extracellular vesicles mediated coagulopathy, inflammation and apoptosis after sepsis
IF: 3.944 -
Biochemical and histological alterations of doxorubicin-induced neurotoxicity in rats: Protective role of luteolin
IF: 3.652Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021)PMID：34766659Reactivity：RatSample Type：brain -
Hydrogen Alleviates Neuronal Injury and Neuroinflammation Induced by Microglial Activation via the Nuclear Factor Erythroid 2-related Factor 2 Pathway in Sepsis-associated Encephalopathy
IF: 3.590Journal： NEUROSCIENCE (2021)PMID：33992722Reactivity：Rat,MouseSample Type：Cell culture supernatant,Tissue homogenate(Brain) -
Dichloroacetic acid-induced dysfunction in rat hippocampus and the protective effect of curcumin
IF: 3.584Journal： METABOLIC BRAIN DISEASE (2021)PMID：33411217Reactivity：RatSample Type：hippocampal tissues
实验操作视频
