Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Exogenous H2S Protects Colon Cells in Ulcerative Colitis by Inhibiting NLRP3 and Activating Autophagy
IF: 3.311 -
Metformin attenuates sepsis-induced neuronal injury and cognitive impairment
IF: 3.288 -
Metformin attenuates LPS-induced neuronal injury and cognitive impairments by blocking NF-κB pathway
IF: 3.288 -
Dehydroevodiamine suppresses inflammatory responses in adjuvant-induced arthritis rats and human fibroblast-like synoviocytes
IF: 3.269 -
Anti-inflammatory activity of 2-((3-(chloromethyl)benzoyl)oxy)benzoic acid in LPS-induced rat model
IF: 3.072Journal： PROSTAGLANDINS & OTHER LIPID MEDIATORS (2021)PMID：33831580Reactivity：RatSample Type：Plasma -
DUSP1 regulates hippocampal damage in epilepsy rats via ERK1/2 pathway
IF: 3.052Journal： JOURNAL OF CHEMICAL NEUROANATOMY (2021)PMID：34562585Reactivity：RatSample Type：Hippocampal tissue -
Melatonin ameliorates myocardial injury by reducing apoptosis and autophagy of cardiomyocytes in a rat cardiopulmonary bypass model
IF: 2.984 -
NF?κB is negatively associated with Nurr1 to reduce the inflammatory response in Parkinson's disease
IF: 2.952 -
Luteolin attenuates doxorubicin-induced derangements of liver and kidney by reducing oxidative and inflammatory stress to suppress apoptosis
IF: 2.903Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2021)PMID：33827303Reactivity：RatSample Type：Tissue homogenate -
Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat.
IF: 2.903
实验操作视频
