Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 2.903Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2021)PMID：34002645Reactivity：RatSample Type：Tissue homogenate(brain) -
Tanshinone IIA improves degranulation of mast cells and allergic rhinitis induced by ovalbumin by inhibiting the PLCγ1/PKC/IP3R pathway
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Achillea millefolium alleviates testicular damage in paclitaxel-intoxicated rats via attenuation of testicular oxido-inflammatory stress and apoptotic responses
IF: 2.775 -
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Melatonin salvages lead-induced neuro-cognitive shutdown, anxiety, and depressive-like symptoms via oxido-inflammatory and cholinergic mechanisms
IF: 2.708 -
Moxibustion against Cyclophosphamide-Induced Premature Ovarian Failure in Rats through Inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent Pyroptosis
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：33613687Reactivity：RatSample Type：Serum -
Si-Miao-Yong-An Decoction Maintains the Cardiac Function and Protects Cardiomyocytes from Myocardial Ischemia and Reperfusion Injury
IF: 2.630 -
The Mitochondrial-Derived Peptide MOTS-c Attenuates Oxidative Stress Injury and the Inflammatory Response of H9c2 Cells Through the Nrf2/ARE and NF-κB Pathways
IF: 2.495 -
Graphene oxide induces dose?dependent lung injury in rats by regulating autophagy
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：33747194Reactivity：RatSample Type：Tissue homogenate
实验操作视频
