Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Emodin ameliorates acute pancreatitis?induced?lung injury by suppressing NLRP3 inflammasome?mediated neutrophil recruitment
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34178130Reactivity：RatSample Type：serum -
Gadolinium chloride pre?treatment reduces the inflammatory response and preserves intestinal barrier function in a rat model of sepsis
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34504589Reactivity：RatSample Type：serum, intestinal tissue -
Activated protein C overexpression suppresses the pyroptosis of subarachnoid hemorrhage model cells by regulating the NLRP3 inflammasome pathway
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34650639Reactivity：RatSample Type：brain tissue -
Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity
IF: 2.315Journal： SOUTH AFRICAN JOURNAL OF BOTANY (2021)Reactivity：RatSample Type：Serum -
miR-484 targeting of Yap1-induced LPS-inhibited proliferation, and promoted apoptosis and inflammation in cardiomyocyte
IF: 2.043Journal： BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2021)PMID：33604630Reactivity：RatSample Type：Cell culture supernatant(H9c2 cells) -
Effects of Achillea millefolium on cisplatin induced ocular toxicity: an experimental study
IF: 1.820 -
Early administration of milrinone ameliorates lung and kidney injury during sepsis in juvenile rats
IF: 1.524 -
Probiotic Saccharomyces boulardii Alleviates Lung Injury by Reduction of Oxidative Stress and Cytokine Response Induced by Supraceliac Aortic Ischemia-Reperfusion Injury in Rats
IF: 1.312Journal： Brazilian Journal of Cardiovascular Surgery (2021)PMID：33355795Reactivity：RatSample Type：serum, Lung tissue -
The effects of vitamin D, fish oil and exercise on cardiovascular parameters in ovariectomized rats
IF: 0.401Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2021)Reactivity：RatSample Type：serum -
Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway
Journal： Journal of Traditional and Complementary Medicine (2021)PMID：34522636Reactivity：RatSample Type：brain
实验操作视频
