Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Lidocaine activates autophagy of astrocytes and ameliorates chronic constriction injury-induced neuropathic pain
IF: 2.476 -
Combination of quercetin and exercise training attenuates depression in rats with 1,2-dimethylhydrazine-induced colorectal cancer: Possible involvement of inflammation and BDNF signalling
IF: 2.431Journal： EXPERIMENTAL PHYSIOLOGY (2020)DOI：10.1113/EP088605PMID：32681548Reactivity：RatSample Type：Serum,Tissue homogenate -
Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats
IF: 2.394 -
The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats
IF: 2.283 -
The novel estrogen receptor GPER1 decreases epilepsy severity and susceptivity in the hippocampus after status epilepticus
IF: 2.274 -
ASIC1a inhibits cell pyroptosis induced by acid-induced activation of rat hepatic stellate cells
IF: 2.231 -
Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats.
IF: 2.173Journal： Therapeutic Advances in Endocrinology and Metabolism (2020)PMID：32782775Reactivity：RatSample Type：Plasma -
Troxerutin attenuates oxygen?glucose deprivation and reoxygenation?induced oxidative stress and inflammation by enhancing the PI3K/AKT/HIF?1α signaling pathway in H9C2 cardiomyocytes
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：32626962Reactivity：RatSample Type：Cell culture supernatant -
Pretreatment with the ALDH2 activator Alda?1 protects rat livers from ischemia/reperfusion injury by inducing autophagy
IF: 2.100 -
Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood
IF: 2.091
实验操作视频
