Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Carbon Monoxide Inhibits the Expression of Proteins Associated with Intestinal Mucosal Pyroptosis in a Rat Model of Sepsis Induced by Cecal Ligation and Puncture
IF: 1.918Journal： MEDICAL SCIENCE MONITOR (2020)PMID：32351244Reactivity：RatSample Type：serum, intestinal tissue -
Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF–κB pathway and activation of Nrf2/ARE pathway in rats
IF: 1.843Journal： INTERNATIONAL UROLOGY AND NEPHROLOGY (2020)PMID：32418008Reactivity：RatSample Type：Serum,Urine,Tissue homogenate -
The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats
IF: 1.805Journal： KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY (2020)Reactivity：RatSample Type：plasma -
The effects of chrysin on lipopolysaccharide-induced sepsis in rats
IF: 1.662Journal： JOURNAL OF FOOD BIOCHEMISTRY (2020)PMID：32614079Reactivity：RatSample Type：lung, liver, kidney -
The protective role of melatonin against the effects of different doses of caffeine on the fetus
IF: 1.270Journal： CELLULAR AND MOLECULAR BIOLOGY (2020)Reactivity：RatSample Type：liver tissue -
Mangiferin ameliorates intracerebroventricular-quinolinic acid-induced cognitive deficits, oxidative stress, and neuroinflammation in Wistar rats
IF: 0.888Journal： INDIAN JOURNAL OF PHARMACOLOGY (2020)PMID：33078731Reactivity：RatSample Type：Tissue homogenate -
Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions
IF: 0.373Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020)Reactivity：RatSample Type：serum -
In vivo exposure to codeine induces reproductive toxicity: role of HER2 and p53/Bcl-2 signaling pathway
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High mobility group box-1 mediates hippocampal inflammation and contributes to cognitive deficits in high-fat high-fructose diet-induced obese rats
IF: 6.170 -
AAV/BBB-Mediated Gene Transfer of CHIP Attenuates Brain Injury Following Experimental Intracerebral Hemorrhage
IF: 5.847
实验操作视频
