Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Co-administration of Luteolin mitigated toxicity in rats' lungs associated with doxorubicin treatment
IF: 3.347Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2020)PMID：33358696Reactivity：RatSample Type：Tissue homogenate -
Impact of improving eating habits and rosmarinic acid supplementation on rat vascular and neuronal system in the metabolic syndrome model
IF: 3.334 -
Palmitic acid stimulates NLRP3 inflammasome activation through TLR4-NF-κB signal pathway in hepatic stellate cells
IF: 3.297Journal： Annals of Translational Medicine (2020)PMID：32309315Reactivity：RatSample Type：Serum,Cell culture supernatant,Tissue homogenate -
Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：33002592Reactivity：RatSample Type：Serum -
Protective effect of Ganoderic acid A on adjuvant-induced arthritis
IF: 3.276 -
Gastrodin alleviates perioperative neurocognitive dysfunction of aged mice by suppressing neuroinflammation
IF: 3.263Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2020)PMID：33220272Reactivity：RatSample Type：Tissue homogenate(Hippocampal tissues) -
Celastrol mitigates high glucose-induced inflammation and apoptosis in rat H9c2 cardiomyocytes via miR-345-5p/growth arrest-specific 6
IF: 3.258 -
Acute aflatoxin B1-induced gastro-duodenal and hepatic oxidative damage is preceded by time-dependent hyperlactatemia in rats
IF: 3.164 -
Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats
IF: 3.139Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2020)PMID：32493122Reactivity：RatSample Type：Tissue homogenate -
H2S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty
IF: 3.139
实验操作视频
