Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats
IF: 2.899 -
Serum thyroid hormones levels are significantly decreased in pregnant rats with acute pancreatitis
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：30286956Reactivity：RatSample Type：Serum -
Rutin attenuates neurobehavioral deficits, oxidative stress, neuro-inflammation and apoptosis in fluoride treated rats
IF: 2.159 -
Sheng-Di-Da-Huang Decoction Inhibited Inflammation Expressed in Microglia after Intracerebral Hemorrhage in Rats
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C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29956782Reactivity：RatSample Type：Synovial fluid,Tissue homogenate -
Mechanism of TangGanJian on nonalcoholic fatty liver disease with type 2 diabetes mellitus
IF: 1.918 -
miR-93-3p alleviates lipopolysaccharide-induced inflammation and apoptosis in H9c2 cardiomyocytes by inhibiting toll-like receptor 4
IF: 1.466Journal： PATHOLOGY RESEARCH AND PRACTICE (2018)PMID：30195636Reactivity：RatSample Type：Cell culture supernatant -
Protective effect of dexpanthenol against cisplatin?induced hepatotoxicity
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402149Reactivity：RatSample Type：Tissue homogenate -
Effects and mechanisms of cold atmospheric plasma on skin wound healing of rats
IF: 1.318Journal： CONTRIBUTIONS TO PLASMA PHYSICS (2018)Reactivity：RatSample Type：skin tissues -
Anti-inflammatory Effects of a Small Molecule Gastrin-Releasing Peptide Receptor Antagonist on Adjuvant-Induced Rheumatoid Arthritis in Rats
IF: 1.258
实验操作视频
