Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Neuroprotective effects of curcumin alleviate lumbar intervertebral disc degeneration through regulating the expression of iNOS, COX?2, TGF?β1/2, MMP?9 and BDNF in a rat model
IF: 1.692Journal： Molecular Medicine Reports (2017)PMID：28901458Reactivity：RatSample Type：intervertebral discs -
Neuroprotective effect of tanshinone IIA weakens spastic cerebral palsy through inflammation, p38MAPK and VEGF in neonatal rats
IF: 1.692 -
A preliminary study on fetal lung injury in a rat model of acute pancreatitis in pregnancy
IF: 1.543 -
Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：28672950Reactivity：RatSample Type：Serum -
Fetal liver injury ameliorated by migration inhibitory factor inhibition in a rat model of acute pancreatitis in pregnancy
IF: 1.099Journal： JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2017)PMID：29227009Reactivity：RatSample Type：serum -
Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats
IF: 0.729 -
Enteric glial cells are susceptible to Clostridium difficile toxin B
IF: 5.694Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2016)PMID：27891552Reactivity：RatSample Type：Cell culture supernatant -
Cinnamaldehyde ameliorates LPS-induced cardiac dysfunction via TLR4-NOX4 pathway: The regulation of autophagy and ROS production
IF: 4.874Journal： JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2016)PMID：27838370Reactivity：RatSample Type：Serum -
Hyperbaric Oxygen Intervention Modulates Early Brain Injury after Experimental Subarachnoid Hemorrhage in Rats: Possible Involvement of TLR4/NF-κ B-Mediated Signaling Pathway
IF: 4.652Journal： CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2016)PMID：27197977Reactivity：RatSample Type：Tissue homogenate -
Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes
IF: 4.492Journal： Oxidative Medicine and Cellular Longevity (2016)PMID：27867451Reactivity：RatSample Type：Tissue homogenate
实验操作视频
