Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Small Molecule Inhibiting Nuclear Factor-kB Ameliorates Oxidative Stress and Suppresses Renal Inflammation in Early Stage of Alloxan-Induced Diabetic Nephropathy in Rat
IF: 3.097Journal： BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY (2016)PMID：27888584Reactivity：RatSample Type：Tissue homogenate -
Venenum Bufonis induces rat neuroinflammation by activiating NF-κB pathway and attenuation of BDNF
IF: 3.055Journal： JOURNAL OF ETHNOPHARMACOLOGY (2016)PMID：27013094Reactivity：RatSample Type：Tissue homogenate -
The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy
IF: 2.730 -
Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study
IF: 1.617Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2016)PMID：27144896Reactivity：RatSample Type：Serum -
Effect of apelin hormone on renal ischemia/reperfusion induced oxidative damage in rats
IF: 0.875 -
Polydatin Alleviates Small Intestine Injury during Hemorrhagic Shock as a SIRT1 Activator
IF: 3.516Journal： Oxidative Medicine and Cellular Longevity (2015)PMID：26301045Reactivity：RatSample Type：Serum -
Transplantation of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation and Neural Damage in Hemorrhagic Stroke
IF: 3.234 -
Osteoprotective Effect of Echinocystic Acid, a Triterpone Component from Eclipta prostrata, in Ovariectomy-Induced Osteoporotic Rats
IF: 3.234 -
Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti?inflammatory effects, and inhibition of the p38?MAPK mechanism
IF: 1.554 -
Protective effect of penehyclidine hydrochloride on lipopolysaccharide-induced acute kidney injury in rat
IF: 0.775
实验操作视频
