Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Atractylodin modulates ASAH3L to improve galactose metabolism and inflammation to alleviate acute lung injury
IF: 4.600Journal： iScience (2024)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF),lung tissue -
Methyltransferase METTL3 regulates neuropathic pain through m6A methylation modification of SOCS1
IF: 4.600Journal： NEUROPHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Lactobacillus rhamnosus GG and Tannic Acid Synergistically Promote the Gut Barrier Integrity in a Rat Model of Experimental Diarrhea via Selective Immunomodulatory Cytokine Targeting
IF: 4.500Journal： MOLECULAR NUTRITION & FOOD RESEARCH (2024)Reactivity：RatSample Type：ileal tissue -
Pharmacological investigation of selected 1,2,4 triazole derivative against ethanol induced gastric ulcer
IF: 4.500Journal： BIOORGANIC CHEMISTRY (2024)Reactivity：RatSample Type：gastric ulcer tissue -
Prevention of dextran sulfate sodium-induced acute enteritis by zinc nanoparticles green-formulated by sodium lignosulfonate
IF: 4.400Journal： INORGANIC CHEMISTRY COMMUNICATIONS (2024)Reactivity：RatSample Type：serum -
Low-molecular-weight heparin ameliorates intestinal barrier dysfunction in aged male rats via protection of tight junction proteins
IF: 4.400 -
Mitigative role of cysteamine against unilateral renal reperfusion injury in Wistar rats
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：serum -
Autophagy Improves Inflammatory Response in Sepsis Accompanied by Changes in Gut Microbiota
IF: 4.400 -
Peptide BG From Bitter Gourd (Momordica Charantia) Improves Adjuvant-Induced Arthritis by Modulating the Necroptosis/Neutrophil Extracellular Traps/Inflammation Axis and the Gut Microbiota
IF: 4.400 -
Mechanism of Astragalus membranaceus (Huangqi, HQ) for treatment of heart failure based on network pharmacology and molecular docking
IF: 4.300
实验操作视频
