Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Long Noncoding RNA SOX2OT Ameliorates Sepsis-Induced Myocardial Injury by Inhibiting Cellular Pyroptosis Through Mediating the EZH2/Nrf-2/NLRP3 Signaling Pathway
IF: 4.200 -
Long-Term Alcohol Exposure Aggravates Ischemic Stroke-Induced Damage by Promoting Pericyte NLRP3 Inflammasome Activation via Pre-Activating the TLR4/NF-κB Pathway in Rats
IF: 4.200 -
Flibanserin conquers murine depressive pseudodementia by amending HPA axis, maladaptive inflammation and AKT/GSK/STAT/BDNF trajectory: center-staging of the serotonergic/adrenergic circuitry
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：hippocampal tissue -
Agmatine modulation of gut-brain axis alleviates dysbiosis-induced depression-like behavior in rats
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：prefrontal cortex (PFC),hippocampus tissue -
Modulation of SIRT6 Related Signaling Pathways of p-AKT/mTOR and NRF2/HO-1 by Memantine Contributes to Curbing the Progression of Tamoxifen/HFD-Induced MASH in Rats
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：liver tissue -
Effects of canagliflozin preconditioning on post-resuscitation myocardial function in a diabetic rat model of cardiac arrest and cardiopulmonary resuscitation
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Andrographis paniculata improves glucose regulation by enhancing insulin sensitivity and upregulating GLUT 4 expression in Wistar rats
IF: 4.000Journal： Frontiers in Nutrition (2024)Reactivity：RatSample Type：Hepatic tissue,muscular tissue -
Botulinum toxin type A-targeted SPP1 contributes to neuropathic pain by the activation of microglia pyroptosis
IF: 3.900Journal： World Journal of Psychiatry (2024)Reactivity：RatSample Type：spinal cord tissue -
Neuroprotective effect of diosmin against chlorpyrifos-induced brain intoxication was mediated by regulating PPAR-γ and NF-κB/AP-1 signals
IF: 3.900Journal： FOOD AND CHEMICAL TOXICOLOGY (2024)Reactivity：RatSample Type：brain tissue -
Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy
IF: 3.900
实验操作视频
