Rat IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：大鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗大鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-1β抗体与结合在包被抗体上的大鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of Bifidobacterium and rosuvastatin on metabolic-associated fatty liver disease via the gut–liver axis
IF: 3.900Journal： Lipids in Health and Disease (2024)Reactivity：RatSample Type：liver tissue -
Metabolomics combined with network pharmacology reveals anti-asthmatic effects of Nepeta bracteata on allergic asthma rats
IF: 3.800Journal： Chinese Herbal Medicines (2024)Reactivity：RatSample Type：serum -
Cryopreserved nanostructured fibrin-agarose hydrogels are efficient and safe hemostatic agents
IF: 3.800 -
Vincamine exerts hepato-protective activity during colon ligation puncture-induced sepsis by modulating oxidative stress, apoptosis, and TNFα/Nrf-2/Keap-1 signaling pathways
IF: 3.800Journal： Scientific Reports (2024)Reactivity：RatSample Type：Hepatic tissue -
The protective effect of vinpocetine against Estradiol-benzoate induced cervical hyperkeratosis in female rats via modulation of SIRT1/Nrf2, and NLRP3 inflammasome
IF: 3.800Journal： Scientific Reports (2024)Reactivity：RatSample Type：Cervical tissue -
Acute exposure to LPS induces cardiac dysfunction via the activation of the NLRP3 inflammasome
IF: 3.800 -
NLRP3 Inhibitor Alleviates Glycemic Variability-Induced Cognitive Impairment in Aged Rats with Type 2 Diabetes Mellitus
IF: 3.800Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：RatSample Type：serum -
The primary studies of epigallocatechin-3-gallate in improving brain injury induced by chronic high-altitude natural environment in rats by 7.0T high-field MR imaging
IF: 3.800Journal： ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2024)Reactivity：RatSample Type：hippocampus tissue -
Unlocking Synergistic Hepatoprotection: Dapagliflozin and Silymarin Combination Therapy Modulates Nuclear Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway in Carbon Tetrachloride-Induced Hepatotoxicity in Wistar Rats
IF: 3.600 -
Testicular toxicity in cisplatin-treated Wistar rats is mitigated by Daflon and associated with modulation of Nrf2/HO-1 and TLR4/NF-kB signaling
IF: 3.600Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)Reactivity：RatSample Type：Testicular tissue
实验操作视频
