Rat IL-4(Interleukin 4) ELISA Kit
中文名称：大鼠白介素4(IL-4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-4抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-4会与包被抗体结合。后依次加入生物素化的抗大鼠IL-4抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-4抗体与结合在包被抗体上的大鼠IL-4结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-4的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Three Birds, One Stone: An Osteo-Microenvironment Stage-Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo-Immunomodulation, Middle Neovascularization, and Later Osteogenesis
IF: 15.1 -
Role of NLRP3 in the exacerbation of ozone-induced allergic rhinitis
IF: 6.8Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID：37742576Reactivity：RatSample Type：nasal lavage fluid -
Berberine-loaded MSC-derived sEVs encapsulated in injectable GelMA hydrogel for spinal cord injury repair
IF: 5.8Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2023)PMID：37536642Reactivity：RatSample Type：peripheral blood -
Mirabegron, dependent on β3-adrenergic receptor, alleviates mercuric chloride-induced kidney injury by reversing the impact on the inflammatory network, M1/M2 macrophages, and claudin-2
IF: 5.6Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)Reactivity：RatSample Type：renal tissue -
Scutellarin attenuates oxidative stress and neuroinflammation in cerebral ischemia/reperfusion injury through PI3K/Akt-mediated Nrf2 signaling pathways
IF: 5.0 -
Ephedra Herb extract ameliorates adriamycin-induced nephrotic syndrome in rats via the CAMKK2/AMPK/mTOR signaling pathway
IF: 4.600 -
Anti-Allergic Effect of Dietary Polyphenols Curcumin and Epigallocatechin Gallate via Anti-Degranulation in IgE/Antigen-Stimulated Mast Cell Model: A Lipidomics Perspective
IF: 4.100 -
Exosomes secreted from bone marrow mesenchymal stem cells suppress cardiomyocyte hypertrophy through Hippo-YAP pathway in heart failure
IF: 2.100 -
Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway
IF: 1.8Journal： GROWTH FACTORS (2023)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells
IF: 11.092
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