Rat IL-5(Interleukin 5) ELISA Kit
中文名称：大鼠白介素5(IL-5)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-5抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-5会与包被抗体结合。后依次加入生物素化的抗大鼠IL-5抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-5抗体与结合在包被抗体上的大鼠IL-5结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-5浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-5的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-5，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Supramolecular gel with enhanced immunomodulatory effects presents a minimally invasive treatment strategy for eosinophilic chronic rhinosinusitis
IF: 10.500Journal： JOURNAL OF CONTROLLED RELEASE (2024)Reactivity：RatSample Type：nasal lavage fluid -
Long-acting anti-inflammatory injectable DEX-Gel with sustained release and self-healing properties regulates TH1/TH2 immune balance for minimally invasive treatment of allergic rhinitis
IF: 10.200Journal： JOURNAL OF NANOBIOTECHNOLOGY (2024)PMID：38321547Reactivity：RatSample Type：nasal lavage fluid (NALF) -
Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：Serum -
Lipidomic profiling of amniotic fluid reveals aberrant fetal lung development and fetal growth disrupted by lipid disorders during gestational asthma
IF: 3.100Journal： JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2024)Reactivity：RatSample Type：alveolar lavage solution -
Role of NLRP3 in the exacerbation of ozone-induced allergic rhinitis
IF: 6.800Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID：37742576Reactivity：RatSample Type：nasal lavage fluid -
Macrophage migration inhibitory factor exacerbates asthmatic airway remodeling via dynamin-related protein 1-mediated autophagy activation
IF: 5.800 -
Tanshinone IIA improves degranulation of mast cells and allergic rhinitis induced by ovalbumin by inhibiting the PLCγ1/PKC/IP3R pathway
IF: 2.903 -
Effect of Src tyrosine kinase on a rat model of asthma
IF: 2.447 -
Lippia javanica (Zumbani) herbal tea infusion attenuates allergic airway inflammation via inhibition of Th2 cell activation and suppression of oxidative stress
Journal： BMC Complementary Medicine and Therapies (2021)PMID：34225706Reactivity：RatSample Type：serum -
Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30520122Reactivity：RatSample Type：Serum,Bronchoalveolar Lavage Fluid
实验操作视频
