Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Preventive effects of melatonin and amifostine on irradiated rats with experimental periodontitis
IF: 2.600 -
Alleviating Effect of a Flower Extract of Styphnolobium japonicum L. on Symptoms of Experimentally Induced Osteoarthritis in Rats
IF: 2.500 -
Autophagy-mediated ferroptosis is involved in development of severe acute pancreatitis
IF: 2.500Journal： BMC GASTROENTEROLOGY (2024)Reactivity：RatSample Type：Serum -
Effect of madecassic acid on retinal oxidative stress, inflammation and Growth Factors in streptozotocin-induced diabetic rats
IF: 2.500Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)Reactivity：RatSample Type：retina tissue -
Pharmacokinetics of seven major components in Aβ1-42-treated rats after oral administration of an aqueous extract of Curculiginis Rhizoma and Epimedii Folium
IF: 2.500Journal： Journal of Natural Medicines (2024)Reactivity：RatSample Type：hippocampus tissue,serum -
Effects of mid?gestational sevoflurane and magnesium sulfate on maternal oxidative stress, inflammation and fetal brain histopathology
IF: 2.400Journal： Experimental and Therapeutic Medicine (2024)Reactivity：RatSample Type：serum -
Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
IF: 2.400Journal： NEUROSCIENCE RESEARCH (2024)Reactivity：RatSample Type：hippocampal tissue -
Formulated Chitosan Microspheres Remodelled the Altered Gut Microbiota and Liver miRNA in Diet-Induced Type-2 Diabetic Rats
IF: 2.400Journal： CARBOHYDRATE RESEARCH (2024)Reactivity：RatSample Type：serum -
The Impact of High-Intensity Interval Training on Insulin Resistance, Oxidative Stress, and Muscle Function in a PCOS Rat Model
IF: 2.400Journal： PHYSIOLOGY & BEHAVIOR (2024)Reactivity：RatSample Type：soleus muscle tissue -
Physical exercise improved the hematological effect of vitamin D in type 2 diabetes mellitus-induced nephrotoxicity in rats
IF: 2.300Journal： Biochemistry and Biophysics Reports (2024)Reactivity：RatSample Type：Renal tissue
实验操作视频
