Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Muscone Abates 1,2-Dimethylhydrazine-Induced Colon Cancer in Rats and HCT-116 Cells Proliferation via Modulation of TNF-α, COX-2, NF-kB, and Phase I Xenobiotic Enzymes
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Mahanimbine Attenuates Ovariectomy-Induced Osteoporosis in Rats by Regulating Bone Turnover Markers and SIRT1-NF-κB Pathway
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Effects of Turmeric, Ginger, Cinnamon, and Garlic Essential Oils on HSP 70, NF-κB, Oxidative DNA Damage, Inflammatory Cytokines, and Oxidative Markers in Broiler
IF: 1.300Journal： Translational Animal Science (2024)Reactivity：ChickenSample Type：serum -
Pandanus amaryllifoius Roxb. Leaves Ethanol Extract Ameliorates Lipid and Proinflammatory Cytokines Profiles in a Rat Model of Dyslipidemia
IF: 1.200Journal： Journal of Pharmacopuncture (2024)Reactivity：RatSample Type：serum -
Pinobanksin Attenuates Cerebral Ischemia–Reperfusion Injury in Rats Through Mitigating the Inflammatory Responses
IF: 0.600Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：Serum,Brain Tissue -
Monotropein Reduces Retinal Inflammation, Oxidative Stress, and Dyslipidemia in Streptozotocin-induced Diabetic Retinopathy in Rats
IF: 0.600Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：serum -
HUB genes transcriptionally regulate lipid metabolism in alveolar type II cells under LPS stimulation
IF: 4.000 -
Ameliorative effects of vitamins-loaded flavoured nanophytosomes fortified with star anise volatile oil against CsA-Induced liver and kidney injury in rats: Application in functional ice cream.
IF: 4.000 -
Sinomenine accelerate wound healing in rats by augmentation of antioxidant, anti-inflammatory, immunuhistochemical pathways.
IF: 4.000 -
RIP1/3-dependent programmed necrosis induces intestinal injury in septic rats: RIP1/3-dependent programmed necrosis induces intestinal injury
IF: 3.700Journal： ACTA BIOCHIMICA ET BIOPHYSICA SINICA (2023)Reactivity：RatSample Type：serum
实验操作视频
