Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Long Non-coding RNA N1LR Protects Against Myocardial Ischemic/Reperfusion Injury Through Regulating the TGF-β Signaling Pathway
IF: 6.050 -
Pantoprazole Attenuates MAPK (ERK1/2, JNK, p38)–NF-κB and Apoptosis Signaling Pathways after Renal Ischemia/Reperfusion Injury in Rats
IF: 5.924Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID：34639009Reactivity：RatSample Type：serum -
Hepatoprotective effect of acetovanillone against methotrexate hepatotoxicity: Role of Keap-1/Nrf2/ARE, IL6/STAT-3, and NF-κB/AP-1 signaling pathways
IF: 5.882Journal： PHYTOTHERAPY RESEARCH (2021)DOI：10.1002/ptr.7355PMID：34939704Reactivity：RatSample Type：liver -
PLGA/PCADK composite microspheres containing hyaluronic acid–chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy
IF: 5.875Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2021)PMID：33493604Reactivity：RatSample Type：Serum -
PARP-1 inhibition attenuates the inflammatory response in the cartilage of a rat model of osteoarthritis
IF: 5.853 -
β-Patchoulene Ameliorates Water Transport and the Mucus Barrier in 5-Fluorouracil-Induced Intestinal Mucositis Rats via the cAMP/PKA/CREB Signaling Pathway
IF: 5.811 -
Salvianolate Ameliorates Osteopenia and Improves Bone Quality in Prednisone-Treated Rheumatoid Arthritis Rats by Regulating RANKL/RANK/OPG Signaling
IF: 5.811 -
Up-regulated microRNA-132 reduces the cognition-damaging effect of sevoflurane on Alzheimer's disease rats by inhibiting FOXA1
IF: 5.736 -
Artesunate ameliorates cigarette smoke-induced airway remodelling via PPAR-γ/TGF-β1/Smad2/3 signalling pathway
IF: 5.631 -
Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism
IF: 5.555
实验操作视频
