Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Accelerating Healing of Excisional Wound with a Hydrogel Containing Hyacinth Bean (Dolichos lablab) Extract in a Rat Model
IF: 0.3Journal： Current Drug Therapy (2024)Reactivity：RatSample Type：skin tissue -
HUB genes transcriptionally regulate lipid metabolism in alveolar type II cells under LPS stimulation
IF: 4.0 -
Ameliorative effects of vitamins-loaded flavoured nanophytosomes fortified with star anise volatile oil against CsA-Induced liver and kidney injury in rats: Application in functional ice cream.
IF: 4.0 -
Sinomenine accelerate wound healing in rats by augmentation of antioxidant, anti-inflammatory, immunuhistochemical pathways.
IF: 4.0 -
RIP1/3-dependent programmed necrosis induces intestinal injury in septic rats: RIP1/3-dependent programmed necrosis induces intestinal injury
IF: 3.7Journal： ACTA BIOCHIMICA ET BIOPHYSICA SINICA (2023)Reactivity：RatSample Type：serum -
Chondroitin Sulphate-Chitosan polyelectrolyte complexes for etorocoxib transdermal delivery: in silico, in?vitro and in?vivo studies
IF: 3.4Journal： PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY (2023)Reactivity：RatSample Type：serum -
Mesenchymal stem cells-derived extracellular vesicle-incorporated H19 attenuates cardiac remodeling in rats with heart failure
IF: 3.3Journal： KAOHSIUNG JOURNAL OF MEDICAL SCIENCES (2023)PMID：37885317Reactivity：RatSample Type：serum -
Melatonin attenuates inflammation and cardiac dysfunction in myocardial infarction by regulating the miRNA-200b-3p/high mobility group box chromosomal protein 1 axis
IF: 2.2Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：serum -
URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats
IF: 2.100Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：heart tissue -
Interleukin-6 Blockers Improve Inflammation-Induced Lipid Metabolism Impairments but Induce Liver Fibrosis in Collagen-Induced Arthritis
IF: 1.900Journal： Endocrine Metabolic & Immune Disorders-Drug Targets (2023)Reactivity：RatSample Type：serum
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