Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway
IF: 1.8Journal： GROWTH FACTORS (2023)Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
Dose-Dependent Effect of Hydrogen Sulfide in Cyclophosphamide-Induced Hepatotoxicity in Rats
IF: 1.2Journal： Turkish Journal of Gastroenterology (2023)Reactivity：RatSample Type：liver homogenates -
Antioxidant, anti-inflammatory, and anti-reprotoxic effects of kaempferol and vitamin E on lead acetate-induced testicular toxicity in male rats
IF: 1.2Journal： Open Veterinary Journal (2023)Reactivity：RatSample Type：Testicular -
Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches
IF: 9.043 -
Effects of Malate Ringer's solution on myocardial injury in sepsis and enforcement effects of TPP@PAMAM-MR
IF: 8.440Journal： Journal of Translational Medicine (2022)Reactivity：RatSample Type：venous blood -
Mild Hypothermia Alleviates CLP-induced Multiple Organ Dysfunction by Mitigating Pyroptosis Through the TLR4/NF-κB/NLRP3 Signaling Pathway
IF: 8.323Journal： ARCHIVES OF MEDICAL RESEARCH (2022)Reactivity：RatSample Type：serum -
Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36499723Reactivity：RatSample Type：kidney -
Methanolic Moringa oleifera leaf extract protects against epithelial barrier damage and enteric bacterial translocation in intestinal I/R: Possible role of caspase 3.
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：RatSample Type：intestinal tissue,hepatic tissue -
ChK1 activation induces reactive astrogliosis through CIP2A/PP2A/STAT3 pathway in Alzheimer's disease
IF: 5.834 -
Polysaccharides from Pseudostellaria heterophylla modulate gut microbiota and alleviate syndrome of spleen deficiency in rats
IF: 4.996
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
