Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Protective Effects of Methoxsalen Supplementation on Chronic Alcohol-induced Osteopenia and Steatosis in Rats
IF: 3.267 -
Gastrodin alleviates perioperative neurocognitive dysfunction of aged mice by suppressing neuroinflammation
IF: 3.263Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2020)PMID：33220272Reactivity：RatSample Type：Tissue homogenate(Hippocampal tissues) -
Celastrol mitigates high glucose-induced inflammation and apoptosis in rat H9c2 cardiomyocytes via miR-345-5p/growth arrest-specific 6
IF: 3.258 -
KGF Is Delivered to Inflammatory and Induces the Epithelial Hyperplasia in Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis Rats
IF: 3.216Journal： Drug Design Development and Therapy (2020)PMID：32021106Reactivity：RatSample Type：intestinal tissue -
Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats
IF: 3.139Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2020)PMID：32493122Reactivity：RatSample Type：Tissue homogenate -
H2S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty
IF: 3.139 -
Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway
IF: 3.056Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID：32671702Reactivity：RatSample Type：Serum -
The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572927Reactivity：RatSample Type：Serum -
Ulinastatin reduces myocardial injury induced by doxorubicin in SD rats
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：33155237Reactivity：RatSample Type：Serum -
The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats.
IF: 2.989
实验操作视频
