Rat IL-6(Interleukin 6) ELISA Kit
中文名称:大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 12.5-800 pg/mL
- 灵敏度: 7.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上,实验时样品(或标准品)中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素,抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 12.5-800 pg/mL |
灵敏度 | 7.5 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells
IF: 2.971Journal: PHARMACEUTICAL BIOLOGY (2020)PMID:32544362Reactivity:RatSample Type:Cell culture supernatant -
The gut microbiota attenuates muscle wasting by regulating energy metabolism in chemotherapy-induced malnutrition rats
IF: 2.967Journal: CANCER CHEMOTHERAPY AND PHARMACOLOGY (2020)PMID:32415349Reactivity:RatSample Type:Serum,Tissue homogenate,Feces -
The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model
IF: 2.907 -
Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies
IF: 2.870 -
Studies on Haloperidol and Adjunctive α-Mangostin or Raw Garcinia mangostana Linn Pericarp on Bio-Behavioral Markers in an Immune-Inflammatory Model of Schizophrenia in Male Rats
IF: 2.849 -
Kukoamine B Ameliorate Insulin Resistance, Oxidative Stress, Inflammation and Other Metabolic Abnormalities in High-Fat/High-Fructose-Fed Rats
IF: 2.842Journal: Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020)PMID:32547146Reactivity:RatSample Type:serum -
Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats
IF: 2.842Journal: Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020)PMID:32606875Reactivity:RatSample Type:Serum -
Inhibition of the fatty acid amide hydrolase changes behaviors and brain catecholamines in a sex-specific manner in rats exposed to chronic unpredictable stress
IF: 2.826 -
Agomelatine prevents indomethacin-induced gastric ulcer in rats
IF: 2.754 -
Ameliorative effect of ozagrel, a thromboxane A2 synthase inhibitor, in hyperhomocysteinemia-induced experimental vascular cognitive impairment and dementia
IF: 2.754Journal: FUNDAMENTAL & CLINICAL PHARMACOLOGY (2020)PMID:33020931Reactivity:RatSample Type:Tissue homogenate