Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Pro-inflammatory cytokines, but not brain- and extracellular matrix-derived proteins, are increased in the plasma following electrically induced kindling of seizures
IF: 2.754 -
Associations of inflammatory markers with impaired left ventricular diastolic and systolic function in collagen-induced arthritis
IF: 2.740 -
Folate receptor-β targeted cholesterol-chitosan nanocarrier for treatment of rheumatoid arthritis: An animal study
IF: 2.734Journal： JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2020)Reactivity：RatSample Type：serum -
Quercetin attenuates naso-sinusal inflammation and inflammatory response in lungs and brain on an experimental model of acute rhinosinusitis in rats
IF: 2.644Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2020)Reactivity：RatSample Type：serum,nasal mucosa,brain,lung -
Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro-inflammatory responses in rats
IF: 2.614Journal： JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2020)PMID：32875596Reactivity：RatSample Type：Tissue homogenate -
Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers
IF: 2.538Journal： Cardiovascular Therapeutics (2020)PMID：32117470Reactivity：RatSample Type：Plasma,Tissue homogenate -
Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase?1/IL?1β Signaling Pathway Inhibition
IF: 2.511Journal： IMMUNOLOGICAL INVESTIGATIONS (2020)PMID：32674625Reactivity：RatSample Type：Cell culture supernatant -
The novel estrogen receptor GPER1 decreases epilepsy severity and susceptivity in the hippocampus after status epilepticus
IF: 2.274 -
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
IF: 2.146Journal： Iranian Journal of Basic Medical Sciences (2020)PMID：33149866Reactivity：RatSample Type：Tissue homogenate -
Pretreatment with the ALDH2 activator Alda?1 protects rat livers from ischemia/reperfusion injury by inducing autophagy
IF: 2.100
实验操作视频
