Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood
IF: 2.091 -
Protective effect of oleuropein on ketamine-induced cardiotoxicity in rats
IF: 2.050Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020)PMID：32383030Reactivity：RatSample Type：Serum -
Carob attenuates nicotine-induced oxidative stress and intratesticular damage in male rats
IF: 1.951 -
Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection
IF: 1.918 -
Protective effect of Alpinetin on rats with chronic obstructive pulmonary disease
IF: 1.797Journal： Food Science & Nutrition (2020)PMID：33312544Reactivity：RatSample Type：Cell culture supernatant -
The effects of chrysin on lipopolysaccharide-induced sepsis in rats
IF: 1.662Journal： JOURNAL OF FOOD BIOCHEMISTRY (2020)PMID：32614079Reactivity：RatSample Type：lung, liver, kidney -
Effect of penehyclidine hydrochloride on IL-6, TNF-α, HIF- 1α and oxidative stress in lung tissue of young rats with acute lung injury
IF: 0.504Journal： TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2020)Reactivity：RatSample Type：Serum -
Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway.
IF: 0.252Journal： International Journal of Clinical and Experimental Pathology (2020)DOI：PMID:32509067PMID：32509067Reactivity：RatSample Type：serum -
Anti-inflammatory activity of Xanthium indicum on carrageenan-induced paw edema in rats
Journal： Advances in Traditional Medicine (2020)Reactivity：RatSample Type：Tissue homogenate -
Polyphenolic-rich extracts of Andrographis paniculata mitigate hyperglycemia via attenuating β-cell dysfunction, pro-inflammatory cytokines and oxidative stress in alloxan-induced diabetic Wistar albino rat
Journal： Journal of Diabetes and Metabolic Disorders (2020)PMID：33553038Reactivity：RatSample Type：serum
实验操作视频
