Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways
IF: 4.936Journal： Oxidative Medicine and Cellular Longevity (2018)PMID：29977458Reactivity：RatSample Type：Cells -
Dual-functional lipid polymeric hybrid pH-responsive nanoparticles decorated with cell penetrating peptide and folate for therapy against rheumatoid arthritis
IF: 4.491Journal： EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2018)PMID：29928978Reactivity：RatSample Type：Plasma -
CIP2A-promoted astrogliosis induces AD-like synaptic degeneration and cognitive deficits
IF: 4.454Journal： NEUROBIOLOGY OF AGING (2018)PMID：30594047Reactivity：RatSample Type：Tissue homogenate,Cell culture medium -
Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats
IF: 3.981Journal： INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY (2018)PMID：30407508Reactivity：RatSample Type：Plasma,Tissue homogenate -
Cold stress provokes lung injury in rats co-exposed to fine particulate matter and lipopolysaccharide
IF: 3.974Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2018)PMID：30384172Reactivity：RatSample Type：Tissue homogenate -
PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro
IF: 3.831Journal： Frontiers in Pharmacology (2018)PMID：30564127Reactivity：ratSample Type：Serum,Cell culture supernatant -
Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the metabolic and immune status in a rat model
IF: 3.755Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2018)PMID：29773169Reactivity：RatSample Type：plasma -
Epigallocatechin gallate attenuates mitochondrial DNA-induced inflammatory damage in the development of ventilator-induced lung injury
IF: 3.610 -
Synergistic and non-synergistic effects of salmon calcitonin and omega - 3 fatty acids on antioxidant, anti-inflammatory, and haematological indices in diabetic rats
IF: 3.457 -
Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity
IF: 3.457
实验操作视频
