Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Java Tea (Orthosiphon stamineus) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study
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SB203580 attenuates acute lung injury and inflammation in rats with acute pancreatitis in pregnancy
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Catechin Attenuates Coronary Heart Disease in a Rat Model by Inhibiting Inflammation
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Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction
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Exosomes derived from miR-146a-modified adipose-derived stem cells attenuate acute myocardial infarction?induced myocardial damage via downregulation of early growth response factor 1
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30362610Reactivity：RatSample Type：Serum,Cell culture supernatant -
MAPKs and NF-κB-mediated acrylamide-induced neuropathy in rat striatum and human neuroblastoma cells SY5Y
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30368882Reactivity：RatSample Type：Tissue homogenate -
Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats
IF: 2.899 -
Protective Effect of Apigenin on Acrylonitrile-Induced Inflammation and Apoptosis in Testicular Cells via the NF-κB Pathway in Rats
IF: 2.884 -
Diabetes escalates knee osteoarthritis in rats: Evidence of adaptive mechanism
IF: 2.776Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2018)PMID：29803977Reactivity：RatSample Type：Serum
实验操作视频
