Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Orthosiphon stamineus (Misai Kucing) ameliorated postmenopausal osteoporosis in rat model
IF: 2.673Journal： MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY (2018)PMID：28926512Reactivity：RatSample Type：serum -
MicroRNA-24 regulates vascular remodeling via inhibiting PDGF-BB pathway in diabetic rat model
IF: 2.498 -
Renal ischemia-reperfusion injury attenuated by splenic ischemic preconditioning
IF: 2.387Journal： European Review for Medical and Pharmacological Sciences (2018)PMID：29687873Reactivity：RatSample Type：serum -
Histopathological and biochemical changes in the development of nonalcoholic fatty liver disease induced by high-sucrose diet at different times
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：30388378Reactivity：RatSample Type：Serum -
Rutin attenuates neurobehavioral deficits, oxidative stress, neuro-inflammation and apoptosis in fluoride treated rats
IF: 2.159 -
Puerarin reduces ischemia/reperfusion-induced myocardial injury in diabetic rats via upregulation of vascular endothelial growth factor A/angiotensin-1 and suppression of apoptosis
IF: 1.922 -
Mechanism of TangGanJian on nonalcoholic fatty liver disease with type 2 diabetes mellitus
IF: 1.918 -
Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling
IF: 1.694Journal： BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018)PMID：30068867Reactivity：RatSample Type：Plasma -
The protective effects of naringin against 5-fluorouracil-induced hepatotoxicity and nephrotoxicity in rats
IF: 1.514Journal： Iranian Journal of Basic Medical Sciences (2018)PMID：29796225Reactivity：RatSample Type：Serum -
miR-93-3p alleviates lipopolysaccharide-induced inflammation and apoptosis in H9c2 cardiomyocytes by inhibiting toll-like receptor 4
IF: 1.466Journal： PATHOLOGY RESEARCH AND PRACTICE (2018)PMID：30195636Reactivity：RatSample Type：Cell culture supernatant
实验操作视频
