Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Bletilla?striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA?induced cirrhotic rats
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30186392Reactivity：RatSample Type：Serum,Tissue homogenate -
Protective effect of dexpanthenol against cisplatin?induced hepatotoxicity
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402149Reactivity：RatSample Type：Tissue homogenate -
Effect of polysaccharides from Vitis vinifera L. on NF-κB/IκB-α signal pathway and inflammatory factors in Alzheimer's model rats
IF: 1.227Journal： BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2018)Reactivity：RatSample Type：Serum -
Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation
IF: 0.933 -
Protective Effect of Nigella Sativa in an Animal Model of Colon Anastomosis With Ischemia/Reperfusion Injury
IF: 0.576Journal： INTERNATIONAL SURGERY (2018)Reactivity：RatSample Type：colon tissue -
Biomarkers of cardio-renal syndrome in uremic myocardiopathy animal model
Journal： Jornal Brasileiro de Nefrologia (2018)Reactivity：RatSample Type：Blood -
Bifidobacterium Infantis Ameliorates Chemotherapy-Induced Intestinal Mucositis Via Regulating T Cell Immunity in Colorectal Cancer Rats
IF: 5.104 -
Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils
IF: 4.593Journal： Oxidative Medicine and Cellular Longevity (2017)PMID：29081886Reactivity：RatSample Type：Cell culture supernatant -
Multifunctional folate receptor-targeting and pH-responsive nanocarriers loaded with methotrexate for treatment of rheumatoid arthritis
IF: 4.300Journal： International Journal of Nanomedicine (2017)PMID：28932117Reactivity：RatSample Type：Serum -
Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways
IF: 4.259
实验操作视频
